Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and thermodynamics. The effects of temperature on kcat/Km for 3alpha-HSD/CR acting on androsterone, 2-decalol, and cyclohexanol show the reactions are entropically favorable but enthalpically unfavorable. Thermodynamic analysis from the temperature-dependent values of Km and kcat shows the binding of the E-NAD+ complex with either 2-decalol or cyclohexanol to form the ternary complex is endothermic and entropy-driven, and the subsequent conversion to the transition state is both enthalpically and entropically unfavorable | Comamonas testosteroni | |
0.16 | - |
2-decalol | pH 10.5, 40°C | Comamonas testosteroni | |
1.7 | - |
2-decalol | pH 10.5, 10°C | Comamonas testosteroni | |
14 | - |
androsterone | pH 10.5, 40°C | Comamonas testosteroni | |
48 | - |
Cyclohexanol | pH 10.5, 40°C | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
androsterone + NAD+ | Comamonas testosteroni | - |
androstanedione + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | P80702 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Comamonas testosteroni |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | rapid equilibrium ordered bi bi kinetic mechanism | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-decalol + NAD+ | - |
Comamonas testosteroni | 2-decanone + NADH + H+ | - |
r | |
androsterone + NAD+ | - |
Comamonas testosteroni | androstanedione + NADH + H+ | - |
r | |
cyclohexanol + NAD+ | - |
Comamonas testosteroni | cyclohexanone + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Comamonas testosteroni |
3alpha-HSD/CR | - |
Comamonas testosteroni |
3alpha-hydroxysteroid dehydrogenase | - |
Comamonas testosteroni |
3alpha-hydroxysteroid dehydrogenase/carbonyl reductase | - |
Comamonas testosteroni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | assay at | Comamonas testosteroni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
Cyclohexanol | pH 10.5, 40°C | Comamonas testosteroni | |
0.91 | - |
2-decalol | pH 10.5, 10°C | Comamonas testosteroni | |
4.3 | - |
2-decalol | pH 10.5, 40°C | Comamonas testosteroni | |
7.2 | - |
androsterone | pH 10.5, 40°C | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
assay at | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Comamonas testosteroni | |
NADH | - |
Comamonas testosteroni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
Cyclohexanol | pH 10.5, 40°C | Comamonas testosteroni | |
0.51 | - |
androsterone | pH 10.5, 40°C | Comamonas testosteroni | |
0.54 | - |
2-decalol | pH 10.5, 10°C | Comamonas testosteroni | |
26.88 | - |
2-decalol | pH 10.5, 40°C | Comamonas testosteroni |